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針對基因組幾何掃描多個引物以查找全部雜交和擴增子。ThermoBLAST發現全部錯誤雜交位點，消滅PCR中的假陽性。
解決引物異性
ThermoBLAST根據大型基因組數據庫自動掃描寡核苷酸，以檢測全部熱力學穩定的命中。與BLAST不同，ThermoBLAST通過考慮以下因素來捕獲關鍵的錯誤雜交命中：
適當的熱力學評分，因為BLAST不會區分GC對和AT對
顯示全部假擴增子
考慮3'的可擴展性和穩定的錯配
ThermoBLAST根據互補性而不是BLAST中的相似性進行評分
ThermoBLAST允許溶液條件、鹽、緩沖液、添加劑和其他實驗因素
PCR假陽性問題
PCR中假陽性的常見原因是由于引物雜交導致假擴增子的形成，而BLAST未檢測到這種情況，因為它使用序列相似性而不是使用匹配和錯配互補性的正確規則對命中進行錯誤評分。此外，很多研究人員無法獲得現代基于基因組的分析設計所需的計算能力或存儲空間。
PCR假陽性的解決辦法
ThermoBLAST CE和ThermoBLAST通過使用BLAST的速度掃描人類基因組或微生物組等全基因組數據庫，同時將適當的熱力學模型應用于錯誤雜交和交叉雜交結果，從而解決了引物和探針錯誤雜交的問題。ThermoBLAST CE的云集成使全部具有計算能力的研究人員能夠捕獲50倍于BLAST的熱力學穩定和可擴展的命中。
預制和可定制的基因組集合
ThermoBLAST-CE包含一個龐大的精選和預格式化序列數據庫存儲庫，可以進一步排列成定制的序列播放列表。下面顯示了一些受歡迎的播放列表?？蛻衄F在可以在不限制內存、存儲或計算能力的情況下構建所需大小的后臺數據庫，這對于那些本地計算能力有限的研究人員來說是一個巨大的痛苦來源。
【英文介紹】
Scans multiple primers against collections of genomes to find all hybridizations and amplicons.
ThermoBLAST finds all mishybridization sites, eliminates false positives in PCR.
Solving Primer Specificity
ThermoBLAST automatically scans oligos against large genome databases to detect all thermodynamically stable hits. Different from BLAST, ThermoBLAST captures the important mishybridization hits by considering the following:
Appropriate thermodynamic scoring, as BLAST doesn't differentiate a GC pair from an AT pair
Displays all false amplicons
Consideration for 3' extensibility and stable mismatches
ThermoBLAST will score based on complementarity as opposed to similarity in BLAST
ThermoBLAST allows for solution conditions, salt, buffers, additives and other experimental factors
The problem of false positives in PCR
The most common cause of false positives in PCR is the formation of false amplicons due to primer mishybridization which is not detected by BLAST because it scores hits incorrectly using sequence similarity rather than using the proper rules for match and mismatch complementarity. Additionally, many researchers do not have access to the computational capacity or storage required of modern genomic-based assay designs.
The solution to false positives in PCR
ThermoBLAST CE and ThermoBLAST solves the problem of primer and probe mishybridization by scanning against whole genome databases such as the human genome or microbiome using the speed of BLAST while applying the proper thermodynamic model to mishybridization and crosshybridization results. The cloud integration of ThermoBLAST CE enables all researchers with the computational power to capture 50X as many thermodynamically stable and extensible hits as BLAST.
Pre-made and customizable genome collections
ThermoBLAST-CE contains a huge repository of curated and pre-formatted sequence databases that can further be arranged into a customized sequence playlist. Some of the most popular playlists are shown below. Customers now have the capability to build a background database as large as they need without restricting memory, storage or computational capacity which is a huge source of pain for those researchers that have limited local computational capacity.
