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ChromasPro用于將序列讀數組裝成contigs。有一個圖形contig編輯器,顯示對齊的色譜圖。它還可以進行一般的序列分析,如限制性酶圖譜、開放讀碼框搜索和BLAST提交。
ChromasPro適用于高達幾兆的DNA序列組裝項目,以及基本的序列編輯和分析。它能夠組裝來自Sanger測序儀(如ABI)和454和Illumina下一代測序儀的數據,如果有8GB內存的話,至多可組裝1,000,000條序列。ChromasPro具有以下特點:
以Applied Biosystems .ab1, Staden Chromatogram (SCF and ZTR), 454 SFF, FASTA, FASTQ, EMBL, GenBank, SwissProt, GenPept, GCG RSF和純文本格式打開序列。
以.ab1、.scf、.fasta和.fastq格式保存序列。
使用圖形界面組裝重疊的序列,生成共識,并突出顯示模棱兩可的地方供編輯。
使用質量數據自動去除低質量的序列,以提高序列組裝。
使用參考序列作為組裝的支架。
生成限制性位點和片段圖,并列出切割、無切割和片段。
在G+C框架圖的幫助下,繪制開放閱讀框架圖,并一鍵翻譯ORFs。
打印色譜圖、限制性位點和片段圖以及開放閱讀框架圖。
通過NCBI網站進行核苷酸和蛋白質BLAST搜索。
通過與ClustalW對接進行多重比對。
反轉和補充序列和色譜圖。
通過準確匹配或optimal排列搜索序列。
在編輯核苷酸序列時顯示翻譯。
進行反向翻譯并繪制核苷酸退化圖。
繪制蛋白質的親水性和抗原性。
復制色譜圖部分的圖像,以便粘貼到文檔或演示文稿中。
系統要求:
與Windows 7sP1,8,10兼容
【英文介紹】
ChromasPro is for assembly of sequence reads into contigs, with a graphical contig editor which displays aligned chromatograms. It also peforms general sequence analysis such as restriction enzyme mapping, open-reading-frame searches, and BLAST submission.
ChromasPro is suitable for DNA sequence assembly projects up to a few megabases, and basic sequence editing and analysis. It is able to assemble data from Sanger sequencers such as ABI, and 454 and Illumina next-generation sequencers, with up to 1,000,000 sequences if 8 Gb RAM is available. ChromasPro has the following features:
Open sequences in Applied Biosystems .ab1, Staden Chromatogram (SCF and ZTR), 454 SFF, FASTA, FASTQ, EMBL, GenBank, SwissProt, GenPept, GCG RSF and plain text formats.
Save sequences in .ab1, .scf, .fasta and .fastq formats.
Assemble overlapping sequences using a graphical interface, generate a consensus and highlight ambiguities for editing.
Use quality data to automatically remove low quality sequence for improved sequence assembly.
Use a reference sequence as a scaffold for assembly.
Generate restriction site and fragment maps, and list cutters, non-cutters and fragments.
Map open reading frames, aided by a G+C frame plot, and translate ORFs with one click.
Print chromatograms, restriction site and fragment maps, and open reading frame maps.
Perform nucleotide and protein BLAST searches through the NCBI web site.
Perform multiple alignments by interfacing with ClustalW.
Reverse & complement sequences and chromatograms.
Search for sequences by exact matching or optimal alignment.
Display translations when editing nucleotide sequences.
Perform reverse translations and plot nucleotide degeneracy.
Plot hydrophilicity and antigenicity of proteins.
Copy an image of a chromatogram section for pasting into documents or presentations.