miltenyi/美天旎 T Cell Activation/Expansion Kit 130-091-441

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品　　牌：miltenyi/美天旎

產品型號：130-091-441

制造廠商：miltenyi/美天旎

適用范圍：全階段

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　　The T Cell Activation/Expansion Kit was developed to activate and expand human T cells. The kit consists of Anti-Biotin MACSiBead Particles and biotinylated antibodies against human CD2, CD3, and CD28. Anti-Biotin MACSiBead Particles loaded with the biotinylated antibodies are used to mimic antigen-presenting cells and activate resting T cells from PBMCs as well as purified T cells. T cell expansion is achieved by culturing and reactivation at day 14 of culture.

　　Product data and images for T Cell Activation/Expansion Kit, human

　　Figures

　　Figure 1

　　Anti-Biotin MACSiBead Particles were loaded with CD2, CD3, and CD28 antibodies. T cells were isolated using the Pan T Cell Isolation Kit. Purified T cells were activated for 72 hours using one loaded Anti-Biotin MACSiBead Particle per two T cells. The negative control experiment was performed without adding MACSiBead Particles. Cells were fluorescently stained using CD4-FITC and CD25-PE or CD4-PE and CD69-FITC.