| Parameter | nProtein A Sepharose 4 Fast Flow |
|---|---|
| Chromatography technique | Antibody affinity chromatography |
| BioProcess resin | Yes |
| Particle size, d50V1 | ~90 μm |
| Matrix | cross-linked agarose, 4%, spherical |
| Ligand Coupling Method | Cyanogen bromide activation |
| Binding Capacity/ml Chromatography Medium | >35 mg human IgG/mL |
| Ligand density | 6 mg Protein A/ml medium |
| Total binding capacity2 | ≥30 mg hIgG/mL chromatography resin |
| pH stability, operational3 | 3–9 |
| pH stability, CIP4 | 2–10 |
| Pressure/flow characteristics5 | 150-250 cm/h at <0.1 MPa in a XK 50/60 column with 5 cm diameter and 25 cm bed height (at 20?C using buffers with the same viscosity as water). |
| Storage | 2 to 8°C, 20% Ethanol |
| Chemical stability | Stable in aqueous buffers commonly used in Protein A chromatography, 6 M guanidine-HCl, 70% ethanol, 3 M NaSCN, 0.1 M glycine (pH 3.0), 2% benzyl alcohol or 20 % ethanol |
| Certificate of Analysis | Yes |
| Pack size | 5 mL |
nProtein A Sepharose 4 Fast Flow is a native protein A resin for purification of monoclonal and polyclonal antibodies.
For the purification of monoclonal and polyclonal antibodies at both laboratory and process scale.
Used in routine commercial polyclonal and monoclonal antibody purification and production.
Free from animal-derived components.
Well suited to immunoprecipitation procedures.
Hydrophilic base matrix ensures low levels of nonspecific binding and low levels of host cell-derived impurities in the elution pool.
Fulfills industrial demands for security of supply, robust performance, and regulatory support.
Use in antibody purification
nProtein A Sepharose 4 Fast Flow is native protein A coupled to the well-established Sepharose 4 Fast Flow base matrix. It is used for commercial production but also for research purposes.The high specificity of the native protein for the Fc region of IgG gives a high purification factor.
