InVivoPlus anti-mouse 4-1BB (CD137)
Clone
Catalog #
Category
3H3
BP0239
InVivoPlus Antibodies
About InVivoPlus anti-mouse 4-1BB (CD137)
The 3H3 monoclonal antibody reacts with mouse 4-1BB, a TNF receptor superfamily member also known as CD137. 4-1BB is a 39 kDa transmembrane protein expressed by T lymphocytes, NK cells, dendritic cells, granulocytes, and mast cells. Upon binding its ligand 4-1BBL, 4-1BB provides costimulatory signals to both CD4 and CD8 T cells through the activation of NF-κB, c-Jun and p38 downstream pathways. The importance of the 4-1BB pathway has been underscored in a number of diseases, including cancer. Agonistic anti-4-1BB antibodies have been reported to induce T cell mediated antitumor immunity. The 3H3 antibody is an agonistic antibody that has been shown to stimulate 4-1BB signaling in vivo.
InVivoPlus anti-mouse 4-1BB (CD137) Specifications
IsotypeRat IgG2a
Recommended Isotype Control(s)InVivoPlus? rat IgG2a isotype control, anti-trinitrophenol
Recommended Dilution BufferInVivoPure? pH 7.0 Dilution Buffer
ImmunogenMouse CD137 human Fc fusion protein
Reported Applications
in vivo 4-1BB stimulation
in vitro 4-1BB stimulation
Formulation
PBS, pH 7.0
Contains no stabilizers or preservatives
*Endotoxin
<1EU/mg (<0.001EU/μg)
Determined by LAL gel clotting assay
*Aggregation
<5%
Determined by DLS
Purity
>95%
Determined by SDS-PAGE
Sterility0.2 μM filtered
ProductionPurified from tissue culture supernatant in an animal free facility
PurificationProtein G
RRIDAB_2687721
Molecular Weight150 kDa
*Murine Pathogen Test Results
Mouse Norovirus: Negative
Mouse Parvovirus: Negative
Mouse Minute Virus: Negative
Mouse Hepatitis Virus: Negative
Reovirus Screen: Negative
Lymphocytic Choriomeningitis virus: Negative
Lactate Dehydrogenase-Elevating Virus: Negative
Mouse Rotavirus: Negative
Theiler’s Murine Encephalomyelitis: Negative
Ectromelia/Mousepox Virus: Negative
Hantavirus: Negative
Polyoma Virus: Negative
Mouse Adenovirus: Negative
Sendai Virus: Negative
Mycoplasma Pulmonis: Negative
Pneumonia Virus of Mice: Negative
Mouse Cytomegalovirus: Negative
K Virus: Negative
StorageThe antibody solution should be stored at the stock concentration at 4°C. Do not freeze.
