Human CD31/PECAM-1 Antibody Summary
Applications
Recommended Concentration
Sample
Western Blot
1 μg/mL
See below
Simple Western
10 μg/mL
See below
Immunocytochemistry
5-15 μg/mL
See below
Knockout Validated
CD31/PECAM?1 was detected in immersion fixed THP?1 human acute monocytic leukemia cell line but is not detected in CD31/PECAM?1 knockout (KO) THP?1 human cell line.
Species Reactivity
Human
Specificity
Detects human CD31 in direct ELISAs and Western blots. In direct ELISAs, less than 5% cross-reactivity with recombinant mouse CD31 is observed.
Source
Polyclonal Sheep IgG
Purification
Antigen Affinity-purified
Immunogen
Chinese hamster ovary cell line CHO-derived recombinant human CD31
Extracellular domain
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
Endotoxin Level
<0.10 EU per 1 μg of the antibody by the LAL method.
Label
Unconjugated