MicroSpin S-400 HR spin columns for DNA purification from unincorporated primers (<32-mers) and nucleotides using spin-column chromatography in applications including desalting, buffer exchange, and primer removal.
Accommodates 25 to 50 μL for post-PCR clean-up prior to cloning or a second amplification reaction, or 25 to 100 μL for all other applications
Convenient: prepacked with Sephacryl? S-400 HR pre-equilibrated in TE (Tris/EDTA) buffer
Ready to use: requires less than 4 min from sample application to collection of purified product
Tested in nickase, single- and double-stranded exonuclease, and RNase assays
MicroSpin S-400 HR spin columns rely on gel filtration for PCR clean-up. Good product yield and purity are obtained with sample volumes from 25-100 μL, and from nanogram to milligram quantities of DNA.
| Parameter | Amersham MicroSpin S-400 HR Columns |
|---|---|
| Application | For the rapid purification of DNA for a wide range of applications, including desalting, buffer exchange, removal of dye terminators from cycle sequencing reactions and removal of labeled nucleotides from DNA labeling reactions |
| Sample volume | < 100 μl |
| Storage | 4°C, TE buffer (10 mM Tris/HCl, 1mM EDTA, pH 7.6) |
| Pack size | 50 columns |
