Recombinant Human M-CSF (C417)
產品說明(Description)
Recombinant Human Macrophage Colony-Stimulating Factor is produced by our Mammalian expression system and the target gene encoding Glu33-Arg255 is expressed with a 6His tag at the C-terminus.
Accession #:P09603
Known as: Macrophage Colony-Stimulating Factor 1; CSF-1; M-CSF; MCSF; Lanimostim; CSF1
制劑（Formulation）
Lyophilized from a 0.2 μm filtered solution of 20mM PB, 150mM NaCl, pH 7.2.
質量控制（Quality Control）
Purity: Greater than 95% as determined by reducing SDS-PAGE.
Endotoxin: Less than 0.1 ng/ug (1 EU/ug) as determined by LAL test.
Bioactivity：Measured in a cell proliferation assay using M?NFS?60 mouse myelogenous leukemia lymphoblast cells. The ED50 for this effect is 1.86ng/mL.
復溶（Reconstitution）
Always centrifuge tubes before opening. Do not mix by vortex or pipetting.
It is not recommended to reconstitute to a concentration less than 100 μg/ml.
Dissolve the lyophilized protein in distilled water.
Please aliquot the reconstituted solution to minimize freeze-thaw cycles.
保存（Storage）
Lyophilized protein should be stored at < -20°C, though stable at room temperature for 3 weeks.Reconstituted protein solution can be stored at 4-7°C for 2-7 days.Aliquots of reconstituted samples are stable at < -20°C for 3 months.
背景（Background）
Macrophage Colony-Stimulating Factors (m-csf) are cytokines that act in hematopoiesis by controlling the production, differentiation, and function of 2 related white cell populations of the blood, the granulocytes and themonocytes-macrophages. CSF-1 promotes the release of proinflammatory chemokines, and thereby plays an important role in innate immunity and in inflammatory processes. It also plays an important role in the regulation of osteoclast proliferation and differentiation, the regulation of bone resorption, and is required for normal bone development. CSF-1 is required for normal male and female fertility and promotes reorganization of the actin cytoskeleton, regulates formation of membrane ruffles, cell adhesion and cell migration. it also plays a role in lipoprotein clearance.
電泳(SDS-PAGE)
參考文獻（References）
Autophagy negative-regulating Wnt signaling enhanced inflammatory osteoclastogenesis from Pre-OCs in vitro[J]. Lili Chen. et al. BIOMEDICINE & PHARMACOTHERAPY.2020.
TRIM24 facilitates antiviral immunity through mediating K63-linked TRAF3 ubiquitination[J]. Qingchen Zhu. et al. JOURNAL OF EXPERIMENTAL MEDICINE.2020.
Identification of compound D2923 as a novel anti-tumor agent targeting CSF1R[J]. Ying-Qiang Liu. et al. Acta Pharmacologica Sinica. 2018.
IL-12+ IL-18 Cosignaling in Human Macrophages and Lung Epithelial Cells Activates Cathelicidin and Autophagy, Inhibiting Intracellular Mycobacterial Growth[J]. Rui Yang. et al. The Journal of Immunology.2018.
FOR RESEARCH USE ONLY
