Use Anti-CTCF Antibody (Rabbit Polyclonal Antibody) validated in ChIP, WB, ChIP-seq to detect CTCF also known as 11-zinc finger protein, CCCTC-binding factor.
Species Reactivity
Key Applications
Host
Format
Antibody Type
H, R, Ca, M, Pm
WB, ChIP-seq, ChIP
Rb
Serum
Polyclonal Antibody
Description
Catalogue Number
07-729
Replaces
MABE547
Brand Family
Upstate
Trade Name
Upstate
Description
Anti-CTCF Antibody
Alternate Names
11 zinc finger transcriptional repressor
11-zinc finger protein
CCCTC-binding factor
CCCTC-binding factor (zinc finger protein)
CTCFL paralog
transcriptional repressor CTCF
Background Information
CTCF is a ubiquitous 11 zinc finger (ZF) protein with highly versatile functions. A transcriptional repressor, CTCF, binds to promoters of vertebrate c-myc gene. It also binds to the PLK and PIM1 promoters. CTCF may prevent the access of transcriptional activators to enhancers. CTCF also acts as a transcriptional activator of APP. It is involved in different aspects of gene regulation including promoter activation or repression, hormone-responsive gene silencing, methylation-dependent chromatin insulation, and genomic imprinting. CTCF may also act as tumor suppressor. CTCF organizes epigenetically controlled chromatin insulators that regulate imprinted genes in soma.
Product Information
Format
Serum
Control
HeLa nuclear extract.
Included Positive Antigen Control:
Catalog # 12-309, HeLa nuclear extract. Add an equal volume of Laemmli reducing sample buffer to 10 μL of extract and boil for 5 minutes to reduce the preparation. Load 20 μg of reduced extract per lane for minigels.
Presentation
Rabbit polyclonal antiserum containing 0.05% sodium azide and 30% glycerol.
