原位細胞3D切割成像平臺

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產品報價： 面議

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產品熱度：加載中

產品型號：CellSurgeon

適用范圍：高教

所在地區：德國

　　原位細胞3D切割成像平臺-CellSurgeon

　　應用領域

　　■  染色體切割

　　■  亞細胞器的實時觀測切割

　　■  原位組織的單細胞分離

　　■  薄組織的顯微切割

　　■  基于激光的光轉染技術

　　CellSurgeon切割原理

　　CellSurgeon將近紅外超短脈沖激光器耦合到顯微鏡中，并利用高數值孔徑物鏡聚焦超短激光脈沖，僅在最小的聚焦體積內產生高強度能量引起多光子吸收，然后以非常精確的方式在活細胞中實現亞細胞水平的細胞結構可視化操作。由于幾乎沒有熱能或機械能傳遞，靠近激光束緊焦點的細胞結構依舊保持完好無損。

　　CellSurgeon的切割方式

　　為何選用CellSurgeon？

　　■  多光子實時成像追蹤

　　■  的3D切割

　　■  無需前處理即可直接切割

　　■  直接的原位切割

　　■  活細胞或組織均可直接切割

　　■  大限度保存生物信息的完整

　　■  能夠兼容多種型號的顯微鏡

　　基本參數

　　■  激光：飛秒近紅外激光，單波長或可變

　　■  掃描器：雙獨立掃描鏡

　　■  掃描精度：700 X 700 ~ 300 X 100

　　■  最大分辨率：700 X 700（1,43 f/s）

　　■  最大掃描速度：300 X 100（10 f/s）

　　■  切割模式：不同波長的2D或3D精準手動或自動切割

　　■  控制器：驅動所有機動單元：顯微鏡、掃描器、 z驅動器、掃描臺以及所有相關配件

　　數據測試

　　■  動脈激光切割和成像

30 fps超短激光脈沖對小鼠血管的損傷

體內激光誘導血栓的三維重建，采用FITC-葡聚糖染色雙光子成像監測激光損傷后血栓的形成情況

　　■  肌動蛋白絲的切割

用飛秒激光切割肌動蛋白細絲

　　■  有絲分裂紡錘體的亞細胞解剖

GBP標記的有絲分裂紡錘體，光漂白(A)和切割消融(B)

　　■  細胞器消融

不同功率激光對核的消融，激光消融前(A)和后(B)

線粒體消融，激光消融前(左)和后(右)

　　■  從細胞到組織的動態觀測與切割

　　CellSurgeon能夠勝任各種類型的切割任務，無論是的染色質還是活體組織，它都能很好地勝任。

　　該設備可以兼容多種型號的顯微鏡，并且支持顯微操作針等配件，能夠在切割后實現對切割部分的轉移。

從細胞團中切除的細胞并用微毛細管將提取細胞切出

白蟻的組織切割

小鼠活體血管切割

　　■  基于激光的原位細胞轉染

　　無論是電轉還是脂質體都需要先將細胞懸浮才能夠進行入轉染，但是Cellsurgeon能夠在原位對細胞進行光穿孔實現細胞的轉染，這種技術對于研究原位的細胞轉染有著重大意義。

使用CellSurgeon對ZMTH3細胞進行轉染pEGFP-C1、pEGFP-HMGA2、pEGFP-HMGB1經過48小時的圖像

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　　部分用戶單位：

　　Bayer HealthCare, Cardiovascular Research

　　Leibniz University Hannover, Institute of Biophysics

　　Leibniz University Hannover, Institute for Quantum Optics-1，-2

　　University of Rostock, Division of Medicine Clinic III, Hematology, Oncology and Palliative Medicine

　　Institute for Bioprocessing and Analytical Measurement Techniques (iba)

　　mfd Diagnostics GmbH